In traditional vaccine preparation, which of the following is not a method used?

In traditional vaccine preparation, various methods are commonly employed to develop effective vaccines that can stimulate an immune response without causing disease. The methods include the use of attenuated viruses, which are live but weakened forms of pathogens that provoke a strong immune response without causing illness. Inactivated vaccines, on the other hand, are made from viruses or bacteria that have been killed or inactivated, ensuring they cannot cause disease but still elicit an immune response. Toxoids are produced from toxins that have been treated to eliminate their harmful effects while retaining their ability to induce immunity.

Insulin, however, is not a method used in vaccine preparation. While it plays a critical role in managing diabetes and is produced using recombinant DNA technology, it does not have a role in traditional vaccine development processes. This distinction highlights the specific nature of vaccine preparation techniques versus pharmaceutical products like insulin, emphasizing that they serve different purposes in medical treatment and prevention.